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Part:BBa_K2411007:Design

Designed by: Abigail Sasdelli   Group: iGEM17_BostonU   (2017-10-01)


Toehold 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We must ensure that the hairpin loop is forming and that the introduction of the toehold to a device does not introduce a frame shift.


Source

This part was synthetically designed by Green et. al. We order the sequence from IDT. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.

References

Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.